Confocal Laser Scanning Microscopy (CLSM)

The Center for Advanced Microscopy offers five high-resolution, point-scanning Confocal Laser Scanning Microscope systems and provides a broad range of CLSM services for both biological and physical sciences.  Each system is designed for observation of a variety of live or fixed biological specimens and a broad range of materials samples.  Instrumentation includes a Nikon A1 CLSM configured with TIRF and STORM, a Nikon C2 CLSM,  a Filter-based Olympus FluoView 1000 CLSM, a Spectral-based Olympus FluoView 1000 CLSM, and a Zeiss 510 Meta ConfoCor3 CLSM.  Click on the microscope of interest for detailed instrument specifications.

A labeled plant cell co-expressing membrane markers of the endoplasmic reticulum, golgi complex, and a soluble endoplasmic reticulum marker. Image taken on the Zeiss Meta at the Center for Advanced Microscopy at Michigan State University. Research from the lab of Dr. Federica Brandizzi.

A labeled plant cell co-expressing membrane markers of the endoplasmic reticulum, golgi complex, and a soluble endoplasmic reticulum marker. Image taken on the Zeiss Meta at the Center for Advanced Microscopy at Michigan State University. Research from the lab of Dr. Federica Brandizzi.

The Center for Advanced Microscopy offers a comprehensive range of advanced imaging applications, including:

  • High-resolution, high-sensitivity confocal imaging in both fluorescence and reflected light modes
  • Multi-channnel fluorescence imaging, including blue fluorescence (excitation 405 nm) through far red fluorescence (excitation 640 nm)
  • High-speed resonance scanning
  • 3D rendering and animation
  • Co-localization analysis
  • Cell counting and image analysis software
  • Live cell imaging and quantitative analysis over time
  • FRAP and FRET imaging and analysis
  • Fluorescence protein imaging, including CPF, GFP, YFP, RFP, and BiFC analysis
  • Spectral imaging
  • High resolution large area scanning
  • Total Internal Reflection Fluorescence (TIRF) microscopy
  • Super Resolution STORM imaging
  • Fluorescence Correlation Spectroscopy (FCS)
  • Extended Depth of Focus (EDF) analysis, Topography analysis, Z height profiling
  • Transmitted light imaging (brightfield, phase contrast, polarized light, DIC)
  • Heated stage incubator (ambient to 99 degrees C)
  • Cooling stage (ambient to -5 degrees C)
  • Heating/cooling/Carbon Dioxide environmental stage incubator

For Confocal Laser Scanning Microscope services contact Melinda Frame, framem@msu.edu, 517-432-2327.

See our Service and User charges page.

For enrollment in NSC-837 Confocal Microscopy Lab, contact Dr. Melinda Frame, framem@msu.edu, 517-432-2327.  This is a limited enrollment course with specific enrollment procedures.   Please see our Courses page for more information.